The feasibility of circulating tumor DNA analysis as a marker of recurrence in triple-negative breast cancer

Triple‑negative breast cancer (TNBC) has a poorer prognosis than other subtypes; therefore, identifying markers of early recurrence is important. The present study aimed to establish liquid biopsy protocol for droplet digital PCR‑based detection frequently mutated genes in patients with TNBC. Tumor DNA from 36 TNBC who relapsed within 2 years after surgical resection was retrospectively analyzed. Somatic mutational profiles were evaluated using targeted sequencing identify and associated molecularly therapies. association between genetic alterations protein phosphorylation investigated immunohistochemical analysis. Recurrent hot spot mutations the plasma monitored over time. Mutation‑specific probes used successfully detect blood samples positive PIK3CA H1047R AKT1 E17K mutations. (14.9%) (25.5%) identified data. Robust AKT S6RP more common tumors background wild‑type AKT1. In conclusion, high‑sensitivity system that also applicable cell‑free DNA. PI3K/AKT pathway revealed be activated harboring mutations; may promising candidate therapy these patients.